{"created":"2023-06-19T07:56:15.606230+00:00","id":825,"links":{},"metadata":{"_buckets":{"deposit":"f45ffe4f-f7b4-49a3-9157-6cb24dcf9afe"},"_deposit":{"created_by":19,"id":"825","owners":[19],"pid":{"revision_id":0,"type":"depid","value":"825"},"status":"published"},"_oai":{"id":"oai:oka-pu.repo.nii.ac.jp:00000825","sets":["15:47:50"]},"author_link":["9","850"],"item_3_alternative_title_5":{"attribute_name":"論文名よみ","attribute_value_mlt":[{"subitem_alternative_title":"キョウショウテン レーザー スキャニング ケンビキョウ ニヨル モウマク シンケイセツ サイボウ ノ サンジゲン ケイタイ ノ カイセキ"}]},"item_3_biblio_info_14":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1995","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"97","bibliographicPageStart":"91","bibliographicVolumeNumber":"2","bibliographic_titles":[{"bibliographic_title":"岡山県立大学保健福祉学部紀要"},{"bibliographic_title":"Bulletin of Faculty of Health and Welfare Science, Okayama Prefectural University","bibliographic_titleLang":"en"}]}]},"item_3_description_11":{"attribute_name":"内容記述(日)","attribute_value_mlt":[{"subitem_description":"複雑な突起形状を有する中枢神経細胞の三次元構造を研究する方法は従来限られており、またそれらの方法によっても非常に時間と手間がかかる上に精度があげられないという問題点があった。しかし、最近になって実用化し普及しつつある共焦点レーザースキャニング顕微鏡(CLSM)はこの問題を解決する可能性を持っている。本研究は、中枢神経系の中でも特に小さく、かつ樹状突起が幅の狭い層に密に展開している網膜神経節細胞の立体構造がCLSM観察によりどの程度解析可能かを検討したものである。Lucifer yellow CH 細胞内注入により網膜神経節細胞を可視化した網膜全伸展標本をCLSMで観察した。その結果、単一細胞の個々の樹状突起や軸索突起を高解像度の投射画像として観察することができた。さらに、細胞の光学的切片像から垂直面への投射像を構成することにより、個々の注入細胞についてその樹状突起の内網状層における展開面を同定し、outer ramifying cell とinner ramifying cellとに明瞭に分類することに成功した。","subitem_description_type":"Other"}]},"item_3_description_12":{"attribute_name":"内容記述(英)","attribute_value_mlt":[{"subitem_description":"A confocal laser scanning microscope (CLSM)has a great advantage for analyzing three-dimensional structure of neuronal cells, which has been difficult and time-consuming with a conventional microscope. We report here that the innovative microscope can provide us new insights for morphological features of mammalian retinal ganglion cells(RGCs)which were injected intracellularly with a fluorescent dye Lucifer yellow. By means of CLSM, both dendritic and axonal processes located in different focal planes can be observed at a single image without any blur. Without sectioning flat mounted retinas vertically, CLSM enabled us to identify either on-type cells or off-type cells clearly and easily onthe basis of their reconstructed vertical image.","subitem_description_type":"Other"}]},"item_3_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.15009/00000814","subitem_identifier_reg_type":"JaLC"}]},"item_3_publisher_20":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"岡山県立大学保健福祉学部"}]},"item_3_relation_22":{"attribute_name":"論文ＩＤ（ＮＡＩＤ）","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"110009424940","subitem_relation_type_select":"NAID"}}]},"item_3_select_1":{"attribute_name":"ページ属性","attribute_value_mlt":[{"subitem_select_item":"P(論文)"}]},"item_3_source_id_13":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN10488896","subitem_source_identifier_type":"NCID"}]},"item_3_source_id_21":{"attribute_name":"ＩＳＳＮ","attribute_value_mlt":[{"subitem_source_identifier":"13412531","subitem_source_identifier_type":"ISSN"}]},"item_3_version_type_19":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"澤井, 元"},{"creatorName":"サワイ, ハジメ","creatorNameLang":"ja-Kana"},{"creatorName":"Sawai, Hajime","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"田内, 雅規"},{"creatorName":"タウチ, マサキ","creatorNameLang":"ja-Kana"},{"creatorName":"Tauchi, Masaki","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2014-12-26"}],"displaytype":"detail","filename":"第2巻 C研究ノート-5.pdf","filesize":[{"value":"13.2 MB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"第2巻 C研究ノート-5","url":"https://oka-pu.repo.nii.ac.jp/record/825/files/第2巻 C研究ノート-5.pdf"},"version_id":"2dbd27f0-2790-4c2e-bba9-bcbce9527072"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"網膜神経節細胞","subitem_subject_scheme":"Other"},{"subitem_subject":"樹状突起","subitem_subject_scheme":"Other"},{"subitem_subject":"軸索","subitem_subject_scheme":"Other"},{"subitem_subject":"共焦点レーザースキャン顕微鏡","subitem_subject_scheme":"Other"},{"subitem_subject":"細胞内注入","subitem_subject_scheme":"Other"},{"subitem_subject":"蛍光色素","subitem_subject_scheme":"Other"},{"subitem_subject":"Retinal ganglion cell","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Dendrite","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Axon","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Confocal laser Scanning microscope","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Intracellular injection","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"共焦点レーザースキャニング顕微鏡による網膜神経節細胞の三次元形態の解析","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"共焦点レーザースキャニング顕微鏡による網膜神経節細胞の三次元形態の解析"},{"subitem_title":"Three-dimensional Structure of Rat Retinal Ganglion Cells Analyzed by Confocal Laser Scanning Microscope","subitem_title_language":"en"}]},"item_type_id":"3","owner":"19","path":["50"],"pubdate":{"attribute_name":"公開日","attribute_value":"2014-12-26"},"publish_date":"2014-12-26","publish_status":"0","recid":"825","relation_version_is_last":true,"title":["共焦点レーザースキャニング顕微鏡による網膜神経節細胞の三次元形態の解析"],"weko_creator_id":"19","weko_shared_id":-1},"updated":"2023-06-19T08:38:12.143336+00:00"}